Australian Trade Minister Mark Vaile said in a statement ahead of his departure for Hong Kong that failure of the talks was "not an option" as the Doha round of trade negotiations had to be finalised in 2006.
"Australian farmers and exporters deserve the chance to compete on a level playing field," he said.
"The Doha Round will determine the international trade rules for the next 25 years, improved market access, reductions in trade-distorting farm subsidies and the elimination of export subsidies are essential outcomes for Australia in agriculture.
"We are sick of having to compete with unfair subsidies provided by some of the world's wealthiest countries.
"Australia is also seeking greater liberalisation of trade in manufactured goods and services, those gains are being put at risk by stubbornness on agriculture," Mr Vaile said.
Agriculture remained the key stumbling block to a deal, with rich nations such as the European Union, Japan and Switzerland refusing to undertake meaningful reform of their farm sectors, he said.
"We believe significant reform in agriculture will deliver the biggest dividend for the developing world, without new trade flows in agriculture we will lose the opportunity to lift millions of people out of poverty.
"We will keep negotiating in Hong Kong to keep the pressure on so that we can finalise the round in 2006," Mr Vaile said.
He added that "there needs to be a sign from developing countries such as Brazil and India that they are committed to liberalisation in manufactured goods and services."
Australia leads the 17-nation Cairns Group of agricultural exporters, comprising Argentina, Australia, Bolivia, Brazil, Canada, Chile, Colombia, Costa Rica, Guatemala, Indonesia, Malaysia, New Zealand, Paraguay, Philippines, South Africa, Thailand and Uruguay.