Local officials told the Kanal 5 television station that a large number of fowl had died in Soniatchne village near Simferopol on the Crimean peninsula.
Preliminary analysis confirmed the presence of bird flu but authorities are unsure if it is the deadly H5N1 strain.
The H5N1 strain has killed more than 70 people in Southeast Asia and China since 2003.
It’s not the first detection of the strain in the Crimea region of Ukraine.
In December 2005, H5N1 was confirmed on the peninsula’s northeastern corner near a migratory site for wild birds.
In the following weeks, almost 70,000 birds were culled as the virus spread to more than 15 villages throughout the region.
Turkey deaths
It comes a day after two teenagers from the same family died from bird flu in Eastern Turkey.
The siblings are first known fatalities of the disease outside of East Asia.
Turkish officials have yet to confirm if the H5N1 strain caused the deaths.
However, a spokeswoman for the World Health Organization (WHO) in Geneva said it was the likely culprit.
If confirmed, the deaths would signify the further westward spread of the disease.
Despite the increasing number of suspect bird flu cases, Turkish Health Minister, Recep Akdag, said an epidemic is unlikely.
"We are not expecting an epidemic in Turkey," Dr Akdag told CNN-Turk.
"There is a risk involved for families and their children who are in close contact with winged animals in unhygienic environments,” he said.
Turkey’s first bird flu victim was 14-year-old Muhammed Ali Kocyigit, who died in a hospital in the city of Van on Sunday.
His 15-year-old sister, Fatma, died at the same hospital on Thursday.
The pair was hospitalised on Saturday with fever, coughing and bleeding from the mouth.
It’s believed they came into contact with chickens that were slaughtered after showing signs of bird flu.