The two men were among a family of five adult Australians who lived in Hong Kong and were travelling on a tour bus that crashed in southern Egypt.
"Two men from the family were killed and three women were hospitalised," a spokesperson from Australia’s Department of Foreign Affairs and Trade (DFAT) said.
The family was with a tour group from Hong Kong.
There were 44 adults on the bus when it crashed between Safaga and Hurghada on the Red Sea coast, the spokesperson said.
Australian consular officials were providing assistance to the
family and their next of kin who have arrived in Eqypt.
DFAT has not released the names of the victims.
Egyptian officials said all 14 people who died, and 29 of the 30 injured, were from Hong Kong.
Another 20 Egyptian pilgrims were reportedly killed just hours later in a separate head-on crash between a bus and car in the village of Al Qaraya, in the country's south.
Six Australians died and 26 were injured three weeks ago when their tour bus flipped on a highway between Cairo and Alexandria.