The sombre service was attended by the province's lieutenant-governor and public security minister, dozens of uniformed police officers, as well as the city's mayor and police chief, who gave one of several touching tributes to Brigadier.
"Today I'm grateful to be able to say goodbye to my partner and tell him that being in the saddle will never be the same," rider Constable Kevin Bradfield said through tears during his eulogy, broadcast on CTV.
Nearby, the horse's bridle and saddle sat alongside its enlarged photo.
The seven-year-old Belgian cross was injured in a hit and run car accident while on patrol in a suburb of Toronto last month and had to be put down.
Constable Bradfield suffered broken ribs and injuries to his neck, back and leg after being thrown from the horse.
The funeral was the second for a fallen police animal this year in Canada.
In February, a German shepherd police dog named Nitro was remembered after falling beneath the wheels of a train while pursuing a suspected car thief in Vancouver, on Canada's Pacific coast.