Mr Larijani, speaking in Algiers, again rejected the offer made by European and US authorities to open talks leading to an incentive package in exchange for a suspension of uranium enrichment activities.
"The issue of uranium enrichment constitutes a major part of those negotiations, so if this part was eliminated then the negotiations would be stripped of all meaning," he said.
Iran however accepted "all constructive and reasonable negotiations (without) any preconditions", he added.
Mr Larijani arrived Monday from Cairo where he had stressed that Tehran would not accept any "threats" from the West over the nuclear issue.
The five permanent UN Security Council members, the United States, Britain, France, Russia and China along with Germany, last week offered Iran a package of incentives in return for reining in its atomic energy program.
Suspending uranium enrichment was the pre-condition for talks on the benefits package.
Pressure increased
The United States has stepped up pressure on Iran to curb its nuclear program as the UN atomic watchdog warned that questions remained over Tehran's nuclear ambitions.
UN nuclear chief Mohamed ElBaradei opened the meeting of the International Atomic Energy Agency saying a more than three-year IAEA probe had failed to resolve "verification issues" over Iran's nuclear work.
"I would continue to urge Iran to provide the cooperation needed to resolve these issues," Mr ElBaradei said.
Despite being unable to certify Iran is not seeking nuclear arms, Mr ElBaradei said he remained "convinced that the way forward lies through dialogue and mutual accommodation among all concerned parties."
Gregory Schulte, the US ambassador to the IAEA, pressed Iran to honour international requests to suspend uranium enrichment, which can make fuel for nuclear power reactors or the raw material for atom bombs.
Tehran should "take advantage of the enormous diplomatic opportunity that lays in front of the Islamic Republic," he said.
Iran says it has a mandate to enrich uranium under the nuclear
Non-Proliferation Treaty as it only wants to use it to make fuel for power generation.
Non-aligned bloc worries Washington
A vigorous debate on Iran but no resolution is expected at this week's IAEA meeting of its 35-nation board of governors, with the Iranian issue expected to come up Wednesday or Thursday.
Diplomats said Washington was fighting to prevent non-aligned states on the IAEA board from issuing a statement supporting Iran's right to uranium enrichment.
A non-aligned diplomat said the bloc was planning a statement that would renew a message first issued May 30 in Malaysia, when the Non Aligned Movement affirmed the right to atomic energy and opposed any attack on nuclear facilities.
The United States wanted the bloc, which numbers some 16 mostly developing nations on the IAEA board, to stick to a February IAEA resolution calling on Iran to suspend uranium enrichment.