Armed with lawn chairs, blankets and plenty of reading material, admirers of the Australian TV star set up camp on a pavement outside a ticket office in the city of Brisbane.
Irwin was killed by the barb of a stingray on September 4 while taking a breaking from filming a documentary.
"I think the stingray didn't know him," said 10-year-old Kiamba Rogers who began lining up outside a ticket sales office early in the morning along with her 17-year-old sister, Tahmea.
"If it knew him, it wouldn't have done it because everybody loves him," Kiamba said.
Thousands of Irwin fans will be able to attend his public memorial service to be held next Wednesday at the 5,500-seater "Crocoseum" in his beloved Australia Zoo wildlife park in northeastern Queensland state.
The cost is a donation to his wildlife charity. Irwin's widow Terri, the couple's eight-year-old daughter Bindi and Irwin's father Bob Irwin will speak during the globally-televised service.
The remembrance ceremony is billed as a celebration of Irwin's life.