Members of the public will be able to listen to and read for free works including "The Origin of Species" and previously unpublished notebooks from his famous voyage on the HMS Beagle.
This five-year journey started in 1831 and took Darwin to South America and Australia, where he collected huge numbers of samples of fossils and living organisms.
It provided the basis for much of his future work and brought him success and celebrity on his return to Britain, although he did not publish "The Origin of Species" until 1859.
The project is being overseen by Dr John van Wyhe of Christ's College, Cambridge, Darwin's alma mater.
Consisting of 50,000 pages of searchable text and 40,000 images of original publications, a university spokesman said it was "the first-ever undertaking of its kind" and "the largest collection of Darwin's writings ever published".
The site is still a work in progress -- Van Wyhe said that, by 2009, the bicentenary of Darwin's birth and 150th anniversary of publication of "The Origin Of Species", he hopes to double the amount of material available on there.
Darwin produced evidence to show that mankind originated through evolutionary change effected by natural selection and his findings are now considered central to our understanding of biology.