Australia's contingent - defending champion Jason Day and in-form Marc Leishman - have been drawn to face off in the same pool at this week's World Golf Championship-Match Play event.
Only one player from each group of four can advance to the Saturday's round of 16 at Austin Country Club in Texas.
Day is top seed in group 3 alongside Leishman, England's Lee Westwood and American Pat Perez.
In the round robin format, which begins Wednesday, world No.3 Day will play Perez in the first round while Leishman faces veteran Westwood.
Two-time WGC-Matchplay winner Day and Leishman, the winner of last week's Arnold Palmer Invitational Leishman, will then square off on Friday.
"Any draw is a tough draw, but match play is a completely different ball game," said Day, who beat South Africa's Louis Oosthuizen in the final in 2016.
"I'm looking forward to defending the WGC Dell Match Play against the best players in the world"
Fresh off winning his first US PGA Tour title in five years at Bay Hill in Florida on Sunday, 33-year-old Leishman said it was unfortunate to be paired against the only other Australian in the field.
"Jason is a great player and good friend, but regardless of who you play, you want to win and I'm sure he'll want to beat me," said Leishman.
Victorian Leishman will draw confidence from a strong record in Presidents Cup match play singles, defeating Jordan Spieth in 2015 and Matt Kuchar in 2013.
"I love match play; I played quite a bit of it growing up and at the Presidents Cup Sunday singles matches I have a good record," said Leishman.
"But that's in the past and I'll have to play solid golf to beat top players like Jason, Pat (Perez) and Lee (Westwood)."
In the other big group matchups, world No.1 Dustin Johnson faces fellowmajor winners in Jimmy Walker, Martin Kaymer and Webb Simpson.
Texan native and world No.6 Jordan Spieth is up against fellow American Ryan Moore as well as Japanese duo Yuta Ikeda and Hideto Tanihara.
World No.2 Rory McIlroy, winner of the 2015 matchplay, is grouped with Argentine Emiliano Grillo, Gary Woodland and Denmark's Soren Kjeldsen.