The team plans to make up for the narrowest of defeats in last year's team time trial (TTT) by winning gold at the 21-28 September UCI Road World Championships in Ponferrada, Spain.
The TTT will be Orica-GreenEDGE's chance to turn the tables on Omega Pharma-QuickStep which has won the past two trade team editions.
The six-man squad announced on Thursday includes four Australians, Luke Durbridge, Brett Lancaster, Michael Hepburn and Damien Howson.
"We're going to the world championships for one reason and that is to win the gold medal," said sport director Matt White.
"We finished third, a little off the pace, in our first attempt two years ago.
"Last year we were agonisingly close, which was incredible when you think the race was over one hour and four minutes and we lost by 0.8 of a second."
The 2014 team, which is rounded out by Canadian Svein Tuft and Jens Mouris from the Netherlands, is the same as last year except Howson comes in for South African Daryl Impey.
"But he (Howson) is no stranger to world championships as he was the world champion last year in the under 23 individual time trial," said White.
Orica-AIS on Thursday also announced its women's time trial team for Sunday.
The team includes four Australians, Annette Edmondson, Melissa Hoskins, Jessie MacLean and Amanda Spratt. Completing the lineup are Swede Emma Johansson and Italian Valentina Scandolara.
Sport director Gene Bates said Orica-AIS was aiming for a medal despite losing Loes Gunnewijk and Shara Gillow due to injury.
"We have a really strong team, we are really motivated and everyone is really fresh in the mind and looking forward to putting a great race together," said Bates.
"I think a podium is a realistic goal for us."
The men's team time trial will be streamed live on the Cycling Central website from 21:30 (9:30pm AEST) on Sunday 21 September. Highlights of the women's race will be available online.
The first five days of the UCI Road World Championships will be live-streamed on the Cycling Central website, while the final two days will be broadcast on SBS ONE and streamed online.