Boels-Dolmans took out the 14th round of the UCI Women's WorldTour covering the 42.5km course in a time of 51 minutes and 43 seconds.
Cervelo-Bigla stopped the clock with the second fastest time at 52 minutes and 19 seconds while Rabobank-Liv rounded out the podium with a one minute and 16 second deficit on the WWT's top ranked team.
Boels-Dolmans rolled down the ramp last and passed the first intermediate checkpoint five seconds down on Cervelo-Bigla's fastest time. But by the second check at 9km from the finish, the dominant team had pulled back the deficit and gained a 39 second advantage.
Finishing the TTT at an average of 49.307 kilometres per hour, director Danny Stam said the team's effort was perfectly paced.
“The pacing strategy was great,” Stam said. “All the girls did an amazing job at the right moments.”
“We have been working towards this since January. There are not many opportunities to train for the TTT during the rest of the season, so it was a focus for our winter training camps.”
While there is still one separate round to go while at Vargarda with the 141km road race on Sunday, the TTT will instill Boels-Dolman with confidence as it eyes off the 2016 UCI Road World Championships TTT.
“It’s a good start on the way to Doha, but there’s a different course in Qatar and more work to come,” Stam said.
“Today shows that the girls came good from Rio. It is nice to start this part of the season with the win.”
“We are definitely going to analyse our ride,” Blaak said. “There are always things to work on and ways to improve. We want to be better before Worlds. We are not there yet, and we need to work for it.
"What’s better than to be the world champion with your own team? That's the main goal and the best way to payback our sponsors and showcase all the hard work we put in all season.”