Ashlee Ankudinoff rode to the individual pursuit silver medal and her sixth career world championships medal at the UCI Track World Championships in Hong Kong.
The 26-year-old was overtaken by American Chloe Dygert, who beat her by seven seconds in the gold medal race but Ankudinoff was still happy with the result and her second silver medal of the week.
"It was nice to have something else to concentrate on other than the team pursuit as all the focus was on that leading up to Rio (Olympic Games)," Ankudinoff said.
"It was great to be given the opportunity to race the individual pursuit here and I am stoked to finish with silver.
"I had my work set out for me against Chloe, but I stuck to my gameplan in qualifying and did a 29 (3minutes 29.554secs).
"Then, in the final, I had nothing to lose, I was up against it, but I just gave it my all and that's all I can ask for."
The women’s Madison made its debut at the 2017 event and Australia were in the medal mix.
Joining Ankudinoff in claiming medals for Australia was the women's madison team, who won bronze after crashes cruelled Amy Cure and Alex Manly's chances of doing better.
Two crashes put them out of contention for the top step of the podium but they still produced a result after Belgium and Great Britain pulled away in the final stages of the race to claim the top gawp places.
"It was a really tough race, we were unlucky to have two crashes, but we fought pretty hard to bring it back, and never gave up," Cure said.
"After the second crash, I did think for a minute that we would be out of the medals.
"But we just kept at it, and we were still in it with a couple of sprints remaining, and I was happy we were able to hold on for that medal."
Australia has now claimed three gold, four silver and three bronze medals ahead of the final day of competition.
Benjamin Thomas of France stormed to gold ahead of New Zealand's Aaron Gate after a thrilling final sprint in the men's omnium
In the men's sprint event, Russia's Denis Dmitriev showed nerves of steel to beat Dutchman Harrie Lavreysen in the final and claim his first world championship gold, with New Zealand's Ethan Mitchell securing the bronze.
Russia claimed two medals in the women's 500m time trial event. Daria Shmeleva won gold and Anastasia Voynova bronze with Germany's 2012 Olympic team sprint champion Miriam Welte taking silver.