RCS Sport and UCI released a statement saying that a total of 571 tests were performed on the 11th and 12th of October.
Team Jumbo-Visma announced that their rider Steven Kruijswick was one of the two to test positive.
Four people from Mitchelton-SCOTT, as well as one each from Team AG2R-La Mondiale and Team Ineos Grenadiers make up the six staff members to test positive.
All eight people "were entrusted to the respective teams’ doctors who ordered their isolation measures," the statement said.
Mitchelton-SCOTT announced that they were withdrawing from Giro d'Italia.
"Mitchelton-SCOTT, in agreement with RCS Sport, has withdrawn its team from the current Giro d’Italia following the results of the first rest day RT-PCR tests," the team said in a statement.
"Upon receiving the results, Mitchelton-SCOTT immediately withdrew its team from the race and will focus on the health of its riders and staff and their safe movement to areas of quarantine."
General Manager Brent Copeland insisted the team had a social responsibility to their riders and staff.
"Thankfully those impacted remain asymptomatic or with mild symptoms, but as an organisation, the health of all of our riders and staff is our main priority and we are now focused on safely transporting them to areas where they are most comfortable to conduct a period of quarantine," Copeland said.
“We wish the RCS and the rest of the peloton a successful finish to this year’s edition of the Giro d’Italia and we look forward to returning in 2021.”