The four teams to make the cut are Cofidis (FRA), Direct Energie (FRA), Fortuneo-Vital Concept (FRA) and Germany's Bora-Argon 18.
The usual 18 WorldTour teams will also line up for the 2-24 July Grand Tour, including Australia's Orica-GreenEDGE and BMC which will be led by Tasmanian Richie Porte.
It will be third consecutive time Bora-Argon 18 will ride the Tour, and with a German start set for 2017, it can also be assured there will be a forth.
“These are great news today," team manager Ralph Denk said. "To be invited for the third time in a row to the worlds biggest cycling event is a big honour, and I want to thank the ASO for their trust in us.
"But it is also a proof that we have presented ourselves very well in the past, and always showed good performances. Now the whole team can focus on the preparations for our highlight – the Tour de France 2016.
"With Sam Bennett we have a very fast talented sprinter for the flat stages. With (Emanuel) Buchmann and (Dominik) Nerz we have two German cards to play when it comes to the big climbs. Nerz has shown in the past that he is also able to play a role in the GC at a Grand Tour, we hope that he is back to full strength this year.
"But also Patrick Konrad has the talent and ability to may surprise in the mountains. But the most important thing now is that all our guys stay healthy. Then we just need some luck to also be spared from any crashes in the first frantic week. If the preparation is good, without any problems, a lot will be possible."
AG2R La Mondiale (FRA)
Astana Pro Team (KAZ)
BMC Racing Team (USA)
Cannondale Pro Cycling Team (USA)
Dimension Data (RSA)
IAM Cycling (SUI)
Lotto Soudal (BEL)
Movistar Team (ESP)
Team Giant-Alpecin (GER)
Team Katusha (RUS)
Team Lotto NL-Jumbo (NED)
Team Sky (GBR)
Wild card teams:
Bora-Argon 18 (GER)
Cofidis, Solutions Crédits (FRA)
Direct Energie (FRA)
Fortuneo-Vital Concept (FRA)