"Some species were already threatened before the fires, but this new analysis also includes other mammal, bird, reptile, frog and crayfish species that were not previously considered to be under threat," Ms Ley said on Tuesday.
The latest report builds on findings published last month that at least 49 threatened species had lost more than 80 per cent of their environment to the fires.
"Many other fire-affected animal species were considered secure and not threatened before the fires, but have now lost much of their habitat and may be imperilled," the report read.
The priority animals, which have the potential to change with further research, were determined on the basis of how much of the habitat had burnt, how endangered they were before the fires, and their traits which influence their vulnerability to fire.
The report singled out the Kangaroo Island dunnart, Pugh's frog and Blue Mountains water skink as at "imminent risk of extinction" due to their previous at-risk status and the significant loss of their habitat.
“We are particularly concerned about species like the Kangaroo Island dunnart and the Blue Mountains water skink, which are that much closer to extinction because of the bushfires,” threatened species commissioner Sally Box said.
Additional species, including the koala, smoky mouse, and giant burrowing frog, were identified as having a "substantial portion" of their habitat affected by fires and in response required emergency intervention to ensure their survival.
Beyond the initial list of 113 animals, the expert panel said it was likely "many other" animals would also require intervention to allow them to properly recover.
Ms Ley said it was still unsafe to enter some of the fire-affected areas, meaning more detailed on-the-ground assessments were needed to establish the full extent of the impact.
So far, however, she said the government was not aware of any species that have gone extinct as a result of the fires.
The number of animals killed in the bushfires that tore through most states and territories in Australia is unknown, but University of Sydney environmental sciences professor Chris Dickman estimated the number was likely higher than one billion.
That figure only takes in birds, reptiles and mammals, excluding bats, frogs, insects and other invertebrates.
The Morrison government has pledged an initial $50 million to fund habitat recovery, including ensuring local wildlife organisations can fund animal treatment, insurance populations, food drops and pest control.
Read the full list of at-risk animals here.