Britain's revolutionary "poo bus" is powered by biomethane gas, produced from human waste.
Britain's first "poo bus", which runs on human and household waste, is set to become a regular service.
Powered by biomethane gas, the Bio-Bus will use waste from more than 32,000 households along its 25km route in Bristol.
Operated by bus company First West of England, the bus will fill up at a site in Avonmouth, Bristol, where sewage and inedible food waste is turned into biomethane gas.
The bus which seats up to 40 people, will operate four days a week from March 25.
If the route proves a success, First will consider introducing more "poo buses".
Managing director James Freeman said the Bio-Bus had generated worldwide attention since it was unveiled.
"The Bio-Bus previously made an appearance running between Bath and Bristol Airport at the end of last year, but it's only actually been used once before in the centre of Bristol itself."
"The very fact that it's running in the city should help to open up a serious debate about how buses are best fuelled, and what is good for the environment," he said.