Mr Trump posed for photos, signed autographs and urged the riders to rev the engines of their bikes.
"Let's hear those engines now," he called out, according to CTV News.
The White House announced the group included military veterans and member of law enforcement agencies, and the Bikers for Trump were prominent fixtures at many of Mr Trump’s campaign rallies – often facing off against anti-Trump protesters.
After posing for selfies and standing with bikers to take the Pledge of Allegiance, Mr Trump addressed the group.
The US president used the photo op to rage against the media and dubbed White House adviser and reality star Omarosa Manigault Newman a “lowlife”.
"Lowlife. She's a lowlife," Mr Trump said.
Earlier, riders had reportedly booed the group of reporters covering the event, before chanting “four more years” as Mr Trump arrived.
Earlier in the day, Mr Trump unleashed on US Attorney General Jeff Sessions and perceived enemies inside the FBI, including former FBI director James Comey.
The president branded them all “clowns and losers” who had hurt “so many great men and women of the FBI”.
Mr Trump called Mr Sessions "scared stiff and Missing in Action."
He also slammed Deputy Attorney General Bruce Ohr, who served as the initial Department of Justice contact for Chris Steele, the former British intelligence officer commissioned by an American political research firm to explore Trump's alleged ties with the Russian government.
Earlier Mr Trump took to Twitter to condemn all violence ahead of the rallies in Charlottesville, before attacking the “fake news media”.