The cadavers and organs have been preserved through plastination, a process through which tissues are essentially turned into plastic.
The exhibit is broken down into several sections, each focused on different physiological functions such as breathing or physical attraction.
It includes a series of embryos and fetuses of varying ages.
The specimens have been sourced from China's Dalian Medical University by the exhibition's US-based organisers, Imagine Exhibitions.
As with similar shows held around the world, some groups such as Doctors Against Forced Organ Harvesting have expressed concerns the bodies may have belonged to people from persecuted Chinese minorities, including members of the Falun Gong.
But Imagine Exhibitions president Tom Zaller says there is "absolutely no basis" for such claims.
"It's a completely ridiculous accusation," Mr Zaller told AAP on Thursday.
Mr Zaller admitted some people have a separate ethical issue about those whose bodies are displayed not having agreed to it.
The bodies were all "unclaimed", Mr Zaller said, meaning they would have been collected by a government entity when the person died before being donated to a medical school.
"This person did not know he would be here. We've never claimed that he would know he would be here," Mr Zaller said, gesturing to a nearby body.
"Just as if you went to the museum and saw the King Tut exhibition, or a mummy exhibition. Those mummies on display, they didn't know they were going to be on display."
Mr Zaller has been involved in similar exhibitions for more than a decade and said their educational benefits were clear.
"What I know now is that because I have displayed these specimens around the world children have become doctors, and that is powerful to me," he said.
Real Bodies has the backing of Australasian Institute of Anatomical Sciences president Gavin Burland.
"An opportunity to come and view the real body and understand the complexity - even I'm in awe, and I've been in the industry for 15 years," he told AAP.
Mr Burland said he was comfortable with the ethics of such exhibitions.
"These bodies would have otherwise been a wasted resource from a scientific perspective."
Real Bodies opens at Sydney's Byron Kennedy Hall at the Entertainment Quarter in Moore Park on Saturday, marking its Australian debut.