Ms Glass says her investigation will consider the conditions under which people have been detained at the tower, the nature and accessibility of official communications with residents, and the nature and appropriateness of restrictions upon people's access to fresh air, exercise, medical care and medical supplies.
As of Thursday, there were 250 coronavirus cases linked to the public housing towers in North Melbourne and Flemington.
The Alfred Street tower was one of nine in those areas to be put into a strict lockdown from 4 to 9 July.
Eight of the towers have reverted to stage three restrictions with the rest of Melbourne, while residents of Alfred Street remain in self-quarantine after it recorded more cases than the other buildings.
Ms Glass said during a global health emergency governments had to act swiftly to save lives, but not at the cost of basic human rights.
"People on the front line are doing an extraordinary job to respond to this crisis and help keep us safe.
"However, there are lessons to be learnt in how governments can do that in a way that protects people's human rights, including access to fresh air, exercise and medical supplies," she said.
Ms Glass added any resident with concerns about their situation can contact her office.
The investigation comes as Victoria on Thursday reached its highest daily number of COVID-19 cases after one week of the new lockdown to bring the coronavirus under control, with 317 new cases.
It marked Australia's biggest daily increase since the start of the pandemic.
Two men in their 80s also succumbed to the disease on Wednesday, bringing the total number of deaths in Victoria to 29 and the national toll to 113.
Metropolitan Melbourne and Mitchell Shire have been in stage three lockdown for a week and are expected to remain under those conditions until 19 August.
Under stage three restrictions people in the locked-down areas are allowed to leave their homes to buy food and supplies, care and caregiving, go to work or school if they cannot do so from home, and exercise.
The government has clarified that people cannot travel for exercise, stating that driving across the city to a favourite walking track is not allowed and people must exercise within their neighbourhoods.
Residents in metropolitan Melbourne are subject to stay-at-home orders and can only leave home for essential work, study, exercise or care responsibilities. People are also advised to wear masks in public.
People in Australia must stay at least 1.5 metres away from others. Check your state’s restrictions on gathering limits.
If you are experiencing cold or flu symptoms, stay home and arrange a test by calling your doctor or contact the Coronavirus Health Information Hotline on 1800 020 080.
News and information is available in 63 languages at sbs.com.au/coronavirus