The event is scheduled for 22 January at the Super-Drome in Adelaide, Australia, with the attempt a part of the Santos Tour Down Under week.
"What better a way to have all out hard work, preparation, data collection, and learnings and capitalize on the number of fans that attend the biggest, best cycling event in Australia, than hold it on the Friday night of the Tour Down Under," O'Donnell .
Her challenge comes four months after American Molly Shaffer Van Houweling set the new mark of 46.273km in Aguascalientes, Mexico.
“It’s going to be a massive challenge now that Molly (Shaffer Van Houweling) has set a new world mark of 46.273kms, but I believe I’m on target and the team around me has supported and encouraged me to no end," she said.
"Everything’s going to plan, and I’m hoping for a big crowd in Adelaide as it’s being staged during the Santos Tour Down Under.”
O’Donnell has represented Australia at three UCI Road World Championships and made twelve podium appearances at Australian and Oceania Championships in the past decade.
The 41-year-old has been training for the attempt for twelve months, ever since the UCI modernised and simplified the regulations for the record by authorising the use of bikes that comply with the regulations for endurance track events.
"This has been a pretty scary and invigorating mission to embark on – not least of all because when one puts herself on the line by setting a date for when preparation, logistics, form and equipment need to be at their very best, well…. it becomes very real," O'Donnell said.
"I started thinking about making an attempt on the Hour Record early this year, many months after the UCI changed the regulations to allow modern track bikes.
"Once I had made my decision to embark on this, the enquiries I made, the long list of requests, permissions, endorsements and paperwork necessary to make an official UCI endorsed attempt took a few months.
"Then there was the enormous financial cost of getting put back into the Registered Testing Pool to establish a biological passport and prove to the UCI that I’m a normal human woman and not a machine.
"As I established when I first got on the boards at the DISC velodrome in Melbourne a few months ago, there were a lot of things I had to learn.
"Fortunately, I would be working with motivated and excellent sport scientists, dedicated technical partners and equipment sponsors with experience and Hour-record know how."