The Trans-Pacific Partnership, finalised last October after five years of negotiations, will be formally agreed to by 12 nations in Auckland, including Australia, and two of the world's three largest economies, in the form of the United States and Japan.
Thousands of people are expected to protest against the deal near where the signing will take in Auckland.
Trade Minister Andrew Robb says the agreement, a bone of contention politically in some countries that will sign it, will eliminate 98 percent of tariffs in the region.
Mr Robb says the deal will drive jobs and growth in Australia.
Trade Minister Andrew Robb is confident the Australian government won't be sued under investor-state dispute clauses in the Trans-Pacific Partnership deal.
Mr Robb is in Auckland with counterparts from 11 other nations putting pen to paper on the deal.
"I'm very confident that what is in the TPP which protects public policy on health and environment will ensure that we are safe," he told Sky News on Thursday.
Investor-state dispute settlement clauses allow foreign investors the right to access an international tribunal if they believe actions taken by a host government breach its investment obligations.
Labor holds concerns about the dispute settlement provisions, which allow foreign investors to sue the government if their profits are affected by any Australian law or policy.
Mr Robb insists the government has had enough time to examine the 6000 page trade document ahead of Thursday's signing.
The deal will then go before the parliaments of participating nations.
He said the agreement between 12 countries takes a "noodle bowl of trade rules" and makes them uniform.
Mr Robb said the signing of the TPP will add extra momentum to trade negotiations on the Regional Comprehensive Economic Partnership (RCEP) which includes China.
There are hopes that deal will be struck this year.
