After more than 100 hours of debate across both houses of Parliament and two demanding all-night sittings, Lower House MPs ratified the Andrews Government's amended bills.
The bill will now go to the Governor for royal assent.
Premier Daniel Andrews, who came to support euthanasia after the death of his father last year, said it was an historic day.
"This is a day of reform, a day of compassion, a day of giving control to those who are terminally ill," he said.
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