A straight-sets elimination for teenage wildcard Alex de Minaur has completed a first-round wipe-out for Australia's Davis Cup stars.
De Minaur succumbed 6-2 6-4 6-3 to British 16th seed Kyle Edmund to join Bernard Tomic, Jordan Thompson, Matt Ebden, John Millman and James Duckworth in failing to progress past round one.
Australian No.1 Nick Kyrgios didn't even start after withdrawing on the eve of his much-anticipated first-time confrontation with Tomic with an elbow injury.
Stosur, Gavrilova and Barty are the only two Australian winners so far, with 17th seed Barty claiming a 6-3, 6-1 win to set up a blockbuster clash against Serena Williams, who also progressed in straight sets.
Stosur powered into the second round for the 12th straight year with a 6-2 6-4 victory over former US Open semi-finalist Yanina Wickmayer.
Unseeded for the first time in Paris for the first time in a decade, Stosur clubbed 21 winners, five aces and broke Wickmayer five times in another typically strong start to her 14th Open campaign.
The veteran clay-courter's only lapse came when she dropped serve from 40-15 up at 4-2 in the second set.
But Stosur broke straight back before closing out the match after one hour and 27 minutes.
