The death of 58 penguins in northern Tasmania is being investigated but autopsies suggest most of the birds were killed by dogs.
Carcasses were collected at Low Head Conservation Area earlier this week after tip-offs from the public, the department said in a statement on Wednesday.
It is the second likely dog attack at Low Head in months, after a dozen birds were killed in June.
"We would like to remind dog owners of the need to take responsibility for the animals at all times," Parks and Wildlife Service state coordinator compliance officer Michael Spaulding said.
"Dogs have the capacity to do a lot of damage to penguin colonies in a short period of time."
The department calling for anyone with information to come forward.
Owners can face fines if their animal is found to have taken wildlife and dogs can be put down.
