Holden managing director Mark Bernhard has thanked the company's workers "from the bottom of my heart" as the the car producer closed its Adelaide assembly operations.
My Bernhard says treating workers with dignity and respect was always the company's top priority since the decision was made in 2013 to end local manufacturing.
"Today is about paying tribute to the generations of men and women across Holden and our supplier network who have given so much to our company," he said on Friday.
"Holden is the icon it is today only because of these passionate people.
"On behalf of everyone at Holden, I thank you for your service from the bottom of my heart."
The final Holden Commodore rolled off the production line on Friday as the company's remaining workers celebrated inside the plant.
It brought Holden's total vehicle production in Australia to 7,687,675 since it first began building cars in 1948.
The plant's closure follows the closure of the Toyota plant in Victoria earlier this month, meaning Australia no longer has a car manufacturing industry.