Hospitality Union 'United Voice' mounted the appeal to keep workers' penalty rates for Sundays and public holidays unchanged, after the first round of cuts came into effect last weekend.
Earlier this year, the Fair Work Commission decided Sunday rates be cut in the fast food, hospitality, retail and pharmacy sectors from July 1st.
But the cuts won't be fully implemented until 2019 or 2020, depending on the industry.
Jess Walsh from hospitality union United Voice says they're arguing the Commision's decision failed to take into account the impact on low paid workers.
James Pearson, the Chief Executive of the Australian Chamber of Commerce and Industry, says the union's campaign is scare-mongering and ultimately holding Australian businesses back.