The Senator wore a full Islamic covering ahead of a debate in parliament in Canberra on banning full-face coverings in public.
The move has been criticised by both sides of politics, but she says she is not at all embarrassed by what she did.
She removed it to ask Attorney-General George Brandis whether the government would consider banning the burqa.
His response was scathing.
Prime Minister Malcolm Turnbull says the foundation of Australia's successful multiculturalism is mutual respect.
Professor John Blaxland from the Australian National University agrees, telling the ABC Ms Hanson's stunt was counter-productive.
Professor Martha Augoustinos, from the School of Psychology, Adelaide University, says Pauline Hanson's action was completely offensive.
Silma Ihram, from the Australia Muslim Women's Association, says the action was uninformed.
"We are free to choose how we dress, particularly in Australia, and it's a terrific thing about Australia that it doesn't force people to assimilate but it recognises their cultural diversity. There are many more pressing issues in terms of security than the burqa."
