"I replied to three essential questions and for all of them, the detector confirmed that I was telling the truth," Kreuziger, 28, revealed on his website kreuzigercase.cz.
The cyclist said he replied to the questions -- "Have you taken doping products?", "Have you used blood transfusions to improve your performance?" and "Have you taken EPO (Erythropoietin)?"
The test was carried out in Prague by British specialist Terry Mullins, "I repeat: I'm not a cheat, nor a liar and I've never taken drugs," the Czech rider added.
Kreuziger has already published his biological passport on his website to back his claim to innocence. The International Cycling Union (UCI) sanctioned the Czech for anomalies in his biological passport during two distinct periods, from March to August 2011 and April 2012 to the end of the 2012 Giro d'Italia, when he was riding for the Astana team.
Polygraphs have been shown to be unreliable in scientific studies, and despite often being used by law enforcement authorities in the past, they're now out of vogue.
His current team, Tinkoff, dropped him from last year's Tour de France with his agreement.
The Court of Arbitration for Sport (CAS) reopened a case against Kreuziger in October after the International Cycling Union (UCI) appealed a Czech Olympic Committee decision to clear him of doping charges that had seen him banned from the sport for months.
Krueziger finished fifth in both the 2011 Tour of Italy and 2013 Tour de France, and won the Amstel Gold Race in 2013. The UCI wants Kreuziger banned for between two and four years and all his results since March 2011 erased, along with a fine of 770,000 Euro ($A1.09 million).
