Supported by various AFL clubs, the players will undergo intense training over the coming months in the hopes of preparing for a draft at the end of next year.
They have come from all walks of life -- netballers, soccer players, hairdressers, even doctors.
After training, Tiarna Ernst will go to the Monash Medical Centre in Melbourne's suburban Clayton for the nightshift on the maternity ward.
"I'm in obstetrics and gynaecology, so (I'll be) delivering babies all night. There's not much free time in the day, that's for sure, but I love it. So, I woke up this afternoon, got ready, packed some food for tonight, and off to training I go. And go home tomorrow morning, sleep, and then get up and do it all over again."
Over a hundred women from across the state have been recruited to AFL Victoria's women's academy.
AFL Victoria Female Football Manager Darren Flanigan says the enthusiasm from recruits drafted into the academy has been overwhelming.
"Of the 114 we invited to join, 109 said yes within two days. So it shows that there's a real keenness for the girls to have an opportunity to do some high-performance training."
It's an elite training program that some are calling a game-changer in the push for a national women's AFL league.
Daisy Pearce played in the two women's AFL exhibition matches this year.
But she says the academy is a whole new level for women in the sport.
"It's the first time that we've had an elite program like this, I suppose. We've had versions of it, but it's definitely gone to the next level now. And I guess, after those initial nerves settled down, everyone's just really thirsty to learn."
The opportunity to play at that next level is attracting players from as far afield as Canada.
Kendra Heil played Australian Rules football in Canada, and her love of the game and the chance to play professionally brought her to Australia.
"I think I'd be really disappointed if the level of play was the same as Canada, just because this is where the sport originated. So I'm happy that it's really intense and really exciting, and it's a hard game and there's lots to learn."
The academy has been given access to coaching staff, players and facilities at a number of Melbourne-based AFL clubs.
The hope is to churn out enough elite-level players for a draft at the end of 2016.
The size and scope of the AFL women's league is yet to be decided, but it is estimated 90 players will be coming out of Victoria.
Victorian Women's Football Academy coach Graham Burgen says it is clear not all of the players at the academy can make the cut.
"There's a fair bit of work to be done, and there's a bit of a gap between the top 40 and the next 60. So we're hoping to close that gap, and that's what the academy's all about."
It makes the potential 2017 launch of the AFL women's league almost a reality.
And for player Daisy Pearce, it would be a dream come true.
"I thought I was going to play AFL footy. I thought that was going to be my career, until I got to about 10 or 11 and suddenly realised that all my heroes ... and my dream wasn't going to come true because I was a girl. So now, to be 27 and for that to maybe become a reality is just incredible."
