Tsonga began the second instalment of the match serving at 5-6 in the fifth set and buckled on the eighth point after the resumption, going wide with a forehand to hand 24th seed American Querrey a 6-2 3-6 7-6(5) 1-6 7-5 victory in a match lasting almost three hours.
Frustrated 12th seed Tsonga thrashed a ball high over the Court Two stands after losing and Querrey sympathised.
"I've been in that situation before (coming out to serve to stay in a match) and it's tough," Querrey, who knocked out defending champion Novak Djokovic on his way to the quarter-finals last year, said after his short workout.
"After such a great match it was a bit of an anti-climax in the end, a bit of a bummer."
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Tsonga was philosophical about his abrupt exit.
"That's just tennis," he said.
(Reporting by Martyn Herman; editing by Clare Lovell)
