"Sunday, MRI scans revealed a minor grade 1 strain in the Biceps Femoris. A minor injury, but enough to end what has been a rather tumultuous year for De Grasse."
A different hamstring injury knocked Canada's highest ranked sprinter out of the world athletics championships in London last year.
De Grasse had said he felt a "little grab" in his right hamstring while leading his 200m semi-final in Saturday's race.
The 23-year-old was hoping the injury was just a cramp but tests revealed the strain.
"I will heal quickly from this injury," De Grasse said in the statement.
"I will go be with my daughter and focus on being a dad for the next few weeks as I heal, and I will plan to come back stronger than ever.”
De Grasse and his girlfriend became the parents of a daughter Yuri on June 22.
“2018 will always be the year I look back at fondly as the year my daughter came into the world” De Grasse said.
Along with last year's hamstring injury, De Grasse learned this year he was recovering from a bout of mononucleosis that he had suffered from possibly as far back as December of 2017, which affected his early races.
He delayed his return until April but was unable to perform at the level that made him the Olympic 200m silver medallist and 100m and 4x100m relay bronze medal winner at the 2016 Rio Games.
Holder of lifetime bests of 9.91 seconds in the 100 and 19.80 in the 200, De Grasse's best 100m time this year was 10.15 in his season opener.
He finished third in the 100 at the Canadian championships on Friday, running 10.20.
His 200m season's best was 20.46.
(Reporting by Gene Cherry in Raleigh, North Carolina; Editing by Clare Lovell)
