Neymar has a "small strain" in an adductor muscle in his left leg, Barca said on their website without specifying how long he would be sidelined.
The injury means the Brazil international is unlikely to feature against Leverkusen with Barca already through to the last 16 of Europe's elite club competition as section winners.
Barca have a La Liga game at home to Deportivo La Coruna on Saturday before they head to Japan for the Club World Cup.
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(Writing by Iain Rogers, editing by Ken Ferris)
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