It was the first time the 56 year old has performed at the Brit Awards in two decades, and it was certainly a show stopper.
Just moments into her song 'Living for Love', Madonna, who was wearing a cape, was pulled backwards down a small flight of stairs by a backup dancer.
The cape she wearing failed to come apart from her costume during the dance routine, resulting her embarrasing tumble. To make matters worse, the lyrics she was singing at the time was, "I will carry on".
On Facebook, Madonna thanked her fans for their support, reassuring them that she was fine.
"Armani hooked me up! My beautiful cape was tied too tight! But nothing can stop me and love really lifted me up! Thanks for your good wishes! I'm fine!"
The rest of the night carried on without any mishaps.
Singer-songwriter Ed Sheeran took home the coveted best album prize at Britain's top music honours on Wednesday for "X" at a star-studded Brit Awards show in London.
The singer looked genuinely surprised as he climbed on the stage to pick up the trophy, a pale pink statuette designed by British artist Tracey Emin.
"I didn't expect it... It's been a very good year for British music", he said.
Other winners of the night included Paloma Faith for British female artist and Royal Blood, who defeated popular boy band One Direction to be named the best British band.
Award for the best international female artist went to Taylor Swift, who opened the show. The pop-heavy ceremony also saw performances from Take That, Sam Smith and Madonna.
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