The 35-year-old Roxburgh Park man was taken to a police station on Thursday and charged with one count of murder over her death.
He had been under police guard in hospital since Monday after allegedly killing Ms Manno, 23, in her Mernda bedroom in the early hours of the morning.
He will face Melbourne Magistrates Court on Thursday evening via an online hearing.
The victim's family alleges the man is an "obsessed" former colleague from the Mill Park call centre where she worked.
The family said a restraining order had been filed against the man, but it wasn't enough to protect her.
Her father Tony feels the system let down his daughter.
"It doesn't bring her back but it's got to be changed," he said.
Chris Ridsdale, Ms Manno's boyfriend, posted an emotional online tribute that featured a photo of the smiling couple from just days ago.
It was the first and final photo of the pair together to be uploaded on social media.
"Now it seems like the best thing I can do for her," Mr Ridsdale wrote on Wednesday when republishing the picture.
"To show everyone how much she meant to me and how beautiful she was."
Ms Manno was a university graduate, with family describing her as universally loved and intelligent.
The number of known deaths due to violence against women in Australia so far this year is 45, according to the Counting Dead Women project.
If you or someone you know is impacted by family or domestic violence, call 1800RESPECT on 1800 737 732 or visit 1800RESPECT.org.au. The Men’s Referral Service provides advice for men on domestic violence and can be contacted on 1300 766 491. In an emergency, call 000.
