Trippier, a right back, has now picked up another knock and has returned to the north London club for treatment.
"The right back hasn't trained all week at St George's Park as he continued rehabilitation on an injury sustained playing for Spurs," an FA statement said. "No replacements are planned at this moment in time..."
Group F leaders England travel to Malta on Friday and host Slovakia at Wembley on Monday.
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(Reporting by Aditi Prakash in Bengaluru; editing by Mark Heinrich)
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