"England head coach Eddie Jones has retained 25 players to continue their preparation ahead of Saturday’s Guinness Six Nations match against Wales in Cardiff," said a statement from the England team on Tuesday.
"Maro Itoje (Saracens) will remain at PennyHill Park (England's training base) this week to continue with his rehabilitation."
Chris Ashton had already been ruled out of the game with a calf strain, while prop Mako Vunipola will miss the remainder of the competition with an ankle problem.
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England lead the Six Nations standings on 10 points after two games, leading Wales, who also won their opening games, due to bonus points from their victories over Ireland and France.
Squad
ForwardsLuke Cowan-Dickie (Exeter), Tom Curry (Sale), Ellis Genge (Leicester), Jamie George (Saracens), Nathan Hughes (Wasps), George Kruis (Saracens), Joe Launchbury (Wasps)Courtney Lawes (Northampton), Ben Moon (Exeter)Brad Shields (Wasps), Kyle Sinckler (Harlequins), Billy Vunipola (Saracens), Harry Williams (Exeter), Mark Wilson (Newcastle)
Backs
Joe Cokanasiga (Bath), Elliot Daly (Wasps), Owen Farrell (Saracens) captain, George Ford (Leicester), Jonny May (Leicester), Jack Nowell (Exeter), Dan Robson (Wasps), Henry Slade (Exeter), Ben Te’o (Worcester), Manu Tuilagi (Leicester), Ben Youngs (Leicester)
(Reporting by Richard Martin; Editing by Ken Ferris)
