He will join the Dutch club’s influential striker Nicolas Jorgenson and first-choice goalkeeper Kenneth Vermeer on the sidelines for the Group F match.
Botteghin suffered a knee injury in the league game at home to NAC Breda on Saturday, which Feyenoord surprisingly lost 2-0, the club said on Monday.
Feyenoord lost their opening Champions League group game 4-0 at home to Manchester City and will start as underdogs against their Italian opponents.
(Reporting by Mark Gleeson, Editing by Ed Osmond)
