Hayne missed Kangaroos training on Monday to have scans and team medical staff later confirmed a partial tear to the hamstring tendon.
The winger will be out of action for at least six weeks.
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"I feel empty at the moment," a shattered Hayne said in a statement issued by the ARL.
"It was pretty sore over the weekend but I was hoping it would be all right.
"It means everything to me to play for my country so to miss out like this, I am shattered.
"I just want to wish the boys well for the tournament and now I just have to focus on my rehab and getting ready for next season."
A replacement player has not yet been named.
The Kangaroos play their first match on Sunday night night against papua New Guinea at Parramatta stadium.
