Conte, who is heading to the English Premier League after bidding goodbye to Italy's national team, was cleared in May of sporting fraud by a judge who said the charge was groundless.
Prosecutors said he had been aware of, but had not intervened to prevent, the alleged fixing of a game between Siena and relegation-threatened AlbinoLeffe, which Conte's Siena side lost 1-0.
The challenge from a prosecutor in the northern Italian city of Brescia, near Milan, means there will be an appeals trial. Italy's three-tier legal system means cases often only end when all possible appeals are exhausted.
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Conte, 46, served a four-month touch-line ban imposed in 2012 by Italian soccer federation FIGC in connection with the case. He has always denied any wrongdoing.
(Reporting by Manuela D'Alessandro, writing by Isla Binnie; Editing by Jon Boyle)
