The Bianconeri players were injured in training and medical checks ruled Dybala, the 22-year-old Argentine, out of the game in Munich on Wednesday due to a minor muscle problem.
Italy international Marchisio was diagnosed with a torn calf muscle and will be sidelined for up to 20 days.
Last season's Champions League finalists came from two goals down to draw 2-2 in Turin and must beat Bayern or score three goals or more in a draw to advance to the quarter-finals of Europe's premier club competition.
Juventus, like Bayern, are chasing a treble of titles this season, leading the Serie A race by three points thanks to a near flawless run this year.
They have conceded only one goal in 12 Serie A matches in 2016, keeping an unprecedented 10th successive clean sheet in the 1-0 win over Sassuolo last week.
(Reporting by Karolos Grohmann, editing by Ed Osmond)
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