The 25-year-old Sunderland winger was carried off on a stretcher in Cairo on Sunday when his team lost to Egypt in a warm-up match before the weekend start of the tournament in Gabon, raising an alarm about his availability.
"It's not a serious injury. It's a benign ankle sprain, he will be able to play in Gabon,” the federation said in a statement on its website.
Khazri is one of Tunisia’s best hopes for the tournament despite a lack of game time for his Premier League club over the last few months.
Tunisia face Senegal, Algeria and Zimbabwe in Group B at the Nations Cup. They face Senegal in their opening game on Sunday.
(Reporting by Mark Gleeson in Cape Town; Editing by Pritha Sarkar)
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