Lucy Turnbull has quit as a director of the independent think tank, the Grattan Institute.
The prime minister's wife, who is a former Sydney lord mayor and business executive, has been on the board since December 2012.
The resignation is noted on Malcolm Turnbull's parliamentary pecuniary interest register.
Grattan Institute chief executive John Daley said Mrs Turnbull had decided she could not give sufficient time to the institute, given her other community roles, and so tendered her resignation.
"She had to make a tough call about her priorities," he said.
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