Ms Winfrey revealed on Monday she would be flying the 300 audience members sitting in her Chicago studio Down Under for an eight-day adventure in December.
Tourism Minister Martin Ferguson said on Tuesday the Oprah Winfrey Show, the most popular US talk fest ever, will film at least two episodes in Australia this summer.
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Mr Ferguson said the program's weekly following of 40 million American viewers, and the fact it is screened in 145 countries, meant the trip would put Australia's beauty on the world stage.
"Oprah is a global household name and her star power has the potential to lift Australia's profile as a premier tourist destination," he said in a statement.
Tourism Australia chairman Geoff Dixon said Oprah's Ultimate Australian Adventure theme was perfect for selling the country's tourism experiences to the rest of the world.
"We know Australia is a dream destination for millions of people," he said.
"The partnership with The Oprah Winfrey Show is a real opportunity to show how they can make their dream a reality."
The iconic Sydney Opera House will be transformed into the Sydney "Oprah" House for the occasion.
Episodes of the show will air in the US in early 2011 and then roll out to other countries.
