The Japanese world number seven was beaten 6-4 6-4 by Pliskova in Tokyo and said afterwards that she was suffering from fatigue.
The Wuhan Open, which began on Sunday and ends on Sept. 29, still boasts a strong field despite Osaka's withdrawal, with the top five women's players, including world number one Simona Halep, all taking part.
(Reporting by Simon Jennings in Bengaluru; Editing by Toby Davis)
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