"The players aren't responding," Hourcade told reporters after the 30-12 reverse in Santa Fe on Saturday. "The responsibility is mine. The cycle is complete."
Hourcade took over as coach in 2013 and led Argentina to the 2015 World Cup semi-finals.
But the Pumas have struggled since, failing to beat the traditional rugby powers and this month they lost two in a row to the Welsh, handing them their first test series victory in Argentina since 1999.
Argentina play Scotland on Saturday in Resistencia.
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(Reporting by Andrew Downie and Ramiro Scandolo, editing by Ed Osmond)
