Carlos Tevez struck a league-leading 15th goal for Juventus in a 2-0 win at AC Milan which gave the champions an 11-point lead over title challengers Roma on Sunday.
Roma sit second with a game in hand but are now lamenting a scoreless draw at home to Inter on Saturday which cost them two precious points and a possible first title since 2001.
Spain striker Fernando Llorente and Tevez had been rested for Juve's 2-0 second leg win over Trabzonspor in the last 32 of the Europa League on Thursday.
And despite missing attacking midfielder Arturo Vidal due to suspension, the Bianconeri soaked up the early pressure to dominate and firmly underline their status as favourites for a third consecutive scudetto.
Milan had to reshuffle with Mario Balotelli injured and midfielder Sulley Muntari suspended, meaning a second consecutive start for striker Giampaolo Pazzini, who wore a face mask to protect a nose injury.
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Juventus broke the deadlock two minutes before the interval after an error by Adil Rami allowed Marchisio to send Lichsteiner through to then cut back for unmarked Llorente to slot home from six yards.
Juventus finally underlined their class when Milan made the fatal mistake of giving Tevez time and space on the outside of the area.
The Argentinian collected from the left, took a touch and unleashed a shot which looped over Abbiati before coming off the underside of the crossbar and into the net.
It was Tevez's 15th league goal this season and took him past injured Fiorentina striker Giuseppe Rossi into the lead of the league scoring charts.
Milan's only response was a strike by Urby Emanuelson which Buffon scrambled to parry, but the hosts were lucky not to be punished further on 87 minutes when Paul Pogba fired a low, angled strike that had Abbiati well beaten but spun off the outside of the 'keeper's upright.
In the other late game, a fifth-minute goal from Loik Cana helped visitors Lazio stun Fiorentina 1-0.
Lazio moved up to eighth with Fiorentina failing to close the gap to Napoli in the final available Champions League qualifying spot.
Napoli, who suffered their second consecutive draw when held earlier at relegation-threatened Livorno, remain third at 17 points adrift of Juve but are seven ahead of Fiorentina.
