The Perth flight will leave Melbourne Airport daily at 7.10am (AEST) with a return flight leaving Western Australia at 9.45am (AWST) from September 7.
It's the fourth route the airline has revived since its flight ban ended on August 12.
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The Civil Aviation Safety Authority grounded the airline on July 1, citing concerns over pilot training, safety management and its overall safety systems.
The airline is now flying daily Melbourne-Sydney, Melbourne-Brisbane and Melbourne-Gold Coast return services.
Tiger spokeswoman Vanessa Regan said the airline will announce the resumption of more routes in due course.
She noted the Melbourne-Perth flights will come days ahead of the AFL finals match between Collingwood and West Coast.
Qantas chief executive Alan Joyce meanwhile will inspect the first works to transform the interior of a Boeing 747 on Friday.
The works to date include the fitting of A380 style seats.
Fledgling domestic carrier Air Australia will also unveil its new crew uniforms on Friday.
The airline operates under the name Strategic Airlines on routes including Melbourne-Phuket and Brisbane-Bali, but will become Air Australia from December 2011 and begin flights between Melbourne and Brisbane.
