Goalkeeper Brad Guzan and defender Joleon Lescott also angered fans for playing a game involving chewing gum on the bench as Villa were held to a 1-1 draw with fourth-tier side Wycombe in the FA Cup, according to British media reports.
"We are going to have our rules, our code of conduct and if someone steps outside there are certainly going to be consequences," Di Matteo, who took charge at the club earlier this month, told British media.
"I will have discussions with all the players, not just one or two. At the end of the day, there are rules people have to obey."
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Seven-times English champions Villa finished 20th in the league last season to drop down to the second tier for the first time since 1987.
(Reporting by Ian Rodricks in Bengaluru; Editing by John O'Brien)
