Caroline Wozniacki has lost in her second match since coming back from injury, falling to Anett Kontaveit of Estonia 6-7 (7-5) 6-3 7-5 at the Nottingham Open.
The former top-ranked player from Denmark was playing at a tournament for the first time since rolling her right ankle in late March.
Top-seeded Czech Karolina Pliskova advanced to the quarter finals of the grass-court tournament by beating Anna Tatishvili 6-1, 6-3.
Alison Riske of the United States beat Su-Wei Hsieh of Taiwan 6-2 6-4 and fourth-seeded Monica Puig of Puerto Rico defeated Michelle Larcher de Brito of Portugal 6-4 6-0.
Australia's Ashleigh Barty is in action on Thursday when she takes on Andre Hlavackova of the Czech Republic in the second round.
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