Queensland Premier Annastacia Palaszczuk denies her government is delaying Adani's planned coal mine and says seeking further review of groundwater plans is common.
Adani Mining's chief executive Lucas Dow says the new request came from the Department of Environment and Science (DES) last Friday.
"It appears this process will again go beyond the scope of what our project is required to deliver under regulatory conditions - and, put simply, is another fishing expedition," he said in a statement.
Federal Environment Minister Melissa Price signed off the company's groundwater plans for its proposed central Queensland mine just before the start of the election campaign.
Mr Dow said the approval followed eight months of assessment, which included reviews by CSIRO and Geoscience Australia.
He said the DES had not provided Adani with the scope of the new review, accusing the government of hiding the process behind a veil of secrecy.
"Trying to see what needs to be done to ensure these management plans can be signed off is like trying to see through a brick wall - there is absolute zero transparency," he said.
The new review means Adani can't start construction of the mine, which has been stuck in the courts and approval process for almost a decade.
Ms Palaszczuk said asking for further analysis of the plans was standard procedure.
"These are just processes that every mining company needs to adhere too," she told reporters on Monday.
"It's not just one rule for Adani and one rule for everyone else."
The decision has been welcomed by the Mackay Conservation Group, which says groundwater resources "deserve the strongest protection".
"Adani are having yet another public dummy spit because the government is simply demanding that it complies with Queensland law and won't permanently damage the Great Artesian Basin," campaigner Michael Kane said.
The mine, to be developed in central Queensland's Galilee Basin, has been a political football, with the country divided on the value of the $2 billion project.
It has dogged the federal election campaign and the coalition believes the Queensland Labor government is putting up roadblocks to win over Green preferences in inner-city seats.
This is the second obstacle for the mine in less than two weeks, with the government recently rejecting Adani's plans for managing the endangered black-throated finch on the site.
