The 52-year-old, nicknamed "Long John", birdied three more times on the back nine to equal the course record, showing that his booming drive is still a powerful weapon.
Belgian Pieters, who has battled illness this week, opened with successive birdies and played the course in clinical fashion to put forward his claim for a place in Europe's Ryder Cup team.
The 26-year-old needs a good showing in Prague to press his case for a wildcard from captain Thomas Bjorn for the Ryder Cup, which begins on Sept. 28 at Le Golf National in Paris.
(Reporting by Simon Jennings in Bengaluru, editing by Ed Osmond)
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